IL28B genetic polymorphism in chronic viral hepatitis

Abstract

As is known, parenteral viral hepatitis often occurs with the formation of cirrhosis of the liver. The exact mechanisms leading to the development of adverse outcomes of the disease are unknown. In recent years, the role of genetic determinants has been widely studied in the development of many systemic diseases, including those associated with cirrhosis of the liver.

Aim - to study the polymorphism of the IL28B gene in chronic viral hepatitis B, C, D and to determine the frequency of their occurrence for the possibility of their further use as prognostic markers of the progression of the process.

Material and methods. 127 patients were examined to analyze the frequency of occurrence of the SNP rs8099917 IL28B gene. Of these, there were 40 people with CHB, 23 people with CHD, and 64 patients with CHC with varying degrees of fibrosis. 2 groups of healthy individuals were taken for control: the local population -28 and the European population - 24 people. The SNP genotyping associated with IL28B rs8099917 was performed by the TaqMan method using a set of reagents with a universal PCR mixture (Applied Biosystems, Foster City, CA) and combined with a special solution of primers and samples.

Statistical data processing was carried out with the determination of average values of indicators (M), calculation of standard errors of average values (m). When comparing values in two groups for normally distributed indicators, the Student's t-test and Fisher's exact criterion were used. The differences were considered significant at р<0.05.

Results and discussion. In healthy individuals, the T allele was major, G - minor. The frequency of genotypes differed with a significant prevalence (by 1.8 times) TT (64.3%) of the genotype over the TG (35.7%) genotype in individuals of the local population. In the European population, the difference in the homozygous T allele and the heterozygous G allele was insignificant (45.8 and 54.2%, respectively). In the group of patients with viral hepatitis, an increase in persons with the TT genotype was noted, in contrast to healthy individuals.

Conclusion. Thus, in patients with viral hepatitis, there was a tendency to a higher frequency of occurrence of the TT genotype in contrast to healthy individuals. It is possible that in persons with a significant prevalence of the TT genotype there is a tendency to viral hepatitis diseases and chronization of the process. Genetic analysis can become a promising method of non-invasive diagnosis and prognosis of the outcomes of chronic viral hepatitis.

Keywords:HBV; HDV; HCV; HBsAg; anti-HCV; liver cirrhosis; IL28B

Funding. The study had no sponsor support.

Conflict of interest. The authors declare no conflict of interest. Contribution. The authors contributed equally to this article.

For citation: Abdukadirova M.A., Khikmatullaeva A.S., Ibadullaeva N.S., Musabaev E.I., Rakhimova V.Sh., Yarmukhamedova N.A., Egamova I.N., Lokteva L.M. IL28B genetic polymorphism in chronic viral hepatitis. Infektsionnye bolezni: novosti, mneniya, obu-chenie [Infectious Diseases: News, Opinions, Training]. 2022; 11 (2): 64-8. DOI: https://doi.org/10.33029/2305-3496-2022-11-2-64-68

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CHIEF EDITOR
CHIEF EDITOR
Yushchuk Nikolay Dmitrievich
Academician of the Russian Academy of Sciences, Professor, head of the Infectious Diseases and Epidemiology Department, President of the Moscow State University of Medicine and Dentistry named after A.I. Evdokimov, Board member of the National Scientific Infectiologists? Society.
Вскрытие
Medicine today

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