Detection of agents of natural foci infections

• Evgeniya S. Filimonova
• Evgeny I. Bondarenko
• Elena I. Krasnova
• Veronika V. Provorova
• Elvira V. Krinitsina
• Anna N. Oleinik
• Tatiana G. Burmistrova

Abstract

The aim - the analysis of frequency of detection of the infections, transferred by ticks (ITT), in an epidemic season of 2018 at adults in the Novosibirsk region.

Material and methods. Clinical material from 325 patients with a feverish state at whom in the anamnesis suction a tick is established is investigated. In samples of biological material of patients with the PTsR-RV method carried out detection of genetic markers of causative agents of the infections, given by mites (ITT): a virus of a tick-borne encephalitis (TBE), the causative agent of the ixodic tick-borne borreliosis (ITBB) entering the Borrelia burgdorferi s.l. complex, activator of a tick-borne garapata disease (Borrelia miyamotoi, Rickettsia species, Coxiella burnetii, Francisella tularensis). By means of EIA blood serum of patients investigated on availability of specific antibodies of IgM and IgG to ITT activators.

Results. At 166 (51.1%) patients from among the inspected 325 patients by means of methods of PTsR-RV and EIA markers of ITT activators are revealed. Markers of TBE are revealed at 78 inspected from which at 5 patients TBE RNA, and is found in 73 IgM to TBE. Markers of the ITBB activator are defined at 40 inspected: in blood of 6 patients DNA of borreliya is found, at 36 in samples of blood serum antibodies of the class IgM and IgG are revealed. Borrelia miyamotoi DNA marker is found in 39 patients in blood. Rickettsia spp. DNA markers, characterized later as Rickettsia sibirica DNA, are revealed in blood of 9 patients. In samples of blood of 9 patients availability of Coxiella burnetii DNA is established, at 4 of which in the period of reconvalescence specific antibodies of the class IgG are found.

Conclusion. It is established that in a sales tax the osknovny reason of the fever which developed after suction of a tick were TBE and ITBB, caused as B. burgdorferi s.l., and B. miyamotoi. The Q fever is diagnosed for 9 patients.

Keywords:tick-transmitted infections, polymerase chain reaction, ELISA test

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