A kit of practice strains for mastering laboratory diagnostics of plague

• Elena V. Sazanova
• Tatyana A. Malyukova
• Boyko A.V.
• Natalia I. Vakhrushina
• Yuri A. Popov

Abstract

State policy in the sphere of biological safety provision requires exclusion or cutting the usage of pathogenic microorganisms in technological processes. Thuswise, it is of relevance to conduct scientific research on biological risk mitigation for educational systems through substitution of virulent strains for avirulent ones.

Aim of the study is to design the kit of practice strains, pathogenic for humans Yersinia and Pasteurella, to develop methodological provision and lower the biological risks in the process of specialist training in laboratory diagnostics of plague.

Material and methods. Studies of strains belonging to Yersinia and Pasteurella genus were performed using bacteriological, immunological (EIA, fluorescent antibody technique), molecular-genetic (PCR, plasmid screening), and biological assays.

Results. The kit, comprising the strains Y. pseudotuberculosis, Y. enterocolitica, P. multocida (III-IV groups of pathogenicity) and strains Y. pestis, avirulent and with attenuated virulence (I group of pathogenicity) has been produced.

Conclusion. The set of practice strains is recommended for usage in specialist training course in laboratory diagnostics of plague. Application of the dummy strains allows for minimizing the possibility of laboratory infection of trainees, exercising specified methods of plague investigation to the fullest extent, and acquiring the skills of safe working with PBA of the first group of hazard.

Keywords:Y. pestis, practice strains, specialist training, biological safety, laboratory diagnostics of plague

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