Prospects for the development of immunofluorescence assay rickettsioses and Q fever

• Pantyukhina A.N.
• Tarasevich Irina V.
• Shpynov S.N.

AbstractThe article presents the author's data on the development of rickettsial multiplex diagnostic kit based on the Rickettsia prowazekii and Coxiella burnetti corpuscular antigens, labeled by colloidal semiconductor quantum dots with different fluorescence colors. Diagnostic kit is for the simultaneous detection of antibodies to R. prowazekii and C. burnetti by immunofluorescence reaction of nanoagglyutination. Using of colloidal semiconductor quantum dots for labeling of antigens is will not only increase the number of simultaneously antibodies in reaction, but also made it possible for objectively assess of results and improve the sensitivity of assay. Our future plans are the development of multiplex diagnostic kits for the detection of antibodies to other dangerous pathogens for human.

Keywords:immunofluorescenсе assay, quantum dots, Rickettsia prowazekii, Coxiell burnetii, multiplexing diagnosticum

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