Genetic markers pathogenicity clinical strains of opportunistic enterobacteria and features associated to acute intestinal infections in adults

• Rasima T. Murzabaeva
• A.R. Mavzyutov
• D.N. Dubrovskaya
• Damir A. Valishin

Abstract

Among the causative agents of acute enteric infections (AEI) is a significant role of opportunistic Enterobacteriaceae, which is associated with changes in the pathogenic potential of microorganisms, including at the genetic level.

Aim - to set the frequency of occurrence of gene fragments islands of pathogenic Escherichia coli (PI) as part of the genomes of clinical strains of opportunistic enterobacteria and show the presence and the nature of the relationship with the specified tag and severity of clinical symptoms of acute intestinal infections in adults.

Results. The study involved 123 patients with acute intestinal infections associated with opportunistic enterobacteria, conducted genetic testing strains of Enterobacteriaceae. It is shown that when lung forms of acute enteric infections often detected E. coli (80%, 8 strains), with moderate - Klebsiella pneumoniae (73.8%, 76 strains), while heavy - Proteus spp. (60%, 6 strains). Genes of PI detected in 49 of 123 clinical isolates of opportunistic enterobacteria (39.84%). At the same time the required genetic determinants of pathogenicity were detected in clinical strains of opportunistic enterobacteria in mild forms of the AEI in 8 out of 10 cases (80%, mainly E. coli), in moderate - in 32 of 103 cases (31.1%, preferably K. pneumoniae) and heavy - 9 out of 10 cases of acute intestinal infections (90% more likely to have Proteus spp.). It was found a statistically significant, strongly influenced by the totality genes of PI detected in 49 isolates of opportunistic enterobacteria, the severity of the disease (17%; η²=17.0%; F=12.0; p<0.001) between the frequency of detection of gene fragments hlyA, hlyB, hlyD in clinical strains of opportunistic enterobacteria, the level of fever, duration of abdominal pain and an erythrocyte sedimentation rate value in patients with acute enteric infection identified correlations, suggesting clinical pathogenicity strains opportunistic enterobacteria carrying genes of PI, and their participation in the development of toxic and diarrhea syndrome.

Keywords:acute enteric infection, Enterobacteriaceae, pathogenicity island, clinical manifestations

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