Evaluation of diagnostic significance of complex detection of the tick-borne infection markers in the patients suffered from its bites

• N.V. Yakovchits
• Evgeny I. Andaev
• Evgeny I. Bondarenko
• Yu.N. Trushina
• I.V. Kotova
• G.B. Pyrinova
• T.G. Vetkina
• S.G. Baranova
• N.G. Topychkanova
• V.I. Ofitserov

Abstract

Object - complex laboratory examination of tick-borne infection vectors and the patients with fever after a tick bite was performed using ELISA and polymerase chain reaction (PCR) methods. Significance of the results was estimated for differential diagnostics of the tick-borne infections.

Material and methods. Total 191 Imago ticks (Ixodes persulcatus) and clinical samples from 65 patients with a fever after the tick bites were included in the work. Genetic material of the causative agents was revealed by real time PCR method. Blood sera were examined for presence of the specific antibody to IgM and IgG by ELISA.

Results. It is established that most carriers infected borrelia (51.9±6.8%), including Borrelia miyamotoi 10.4±2.8%. The remaining pathogens are much less common, from 18.1 to 3.6%. In the study of clinical data in 21 cases on the basis of genotype or serological diagnosis was diagnosed with «encephalitis». In 10 patients with antibodies to pathogens borreliosis and/or genetic material of the pathogen, the etiological cause of fever in five was Borrelia miyamotoi.

Conclusion. According to the results of comprehensive laboratory testing, it was found that the main cause of fever after tick bites is infected with tick-borne encephalitis. The detection of Borrelia, despite the high level of contamination of carriers, two times lower.

Keywords:Borrelia miyamotoi, borreliosis, tick-borne infection, returnable fever, rickettsiosis, real time polymerase chain reaction

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